In vitro
Cell culture, cancer cell lines, primary patient cells, PBMC isolation from whole blood,
flow cytometry, apoptosis and cell cycle analyses, Western blot, DNA/RNA extraction and
purification, cDNA synthesis, RT-qPCR, immunofluorescence, trypan blue exclusion, SRB, MTT,
mitochondrial assays (ROS production, MitoTracker, MitoView), analysis of sequencing datasets.
In vivo
Cell line-derived and patient-derived xenograft mice: handling, restraint, injection,
tumor volume, humane methods of killing, bone marrow flushing, organ collection.
C. elegans
Mutant and transgenic strains, drug screening, microscopy, microbiology preparations.
Software & other
Microsoft Office, GraphPad Prism, GuavaSoft, ImageJ, Leica LAS X, XLStat. Protocol development,
experimental design, cross-disciplinary collaboration, supervision and mentoring of students,
writing, and public speaking.